Scientists draw DNA from ancient eggs

© iStockphoto.com/Jan Rysavy

AUSTRALIAN scientists have, in a world first, extracted DNA from the fossilised eggshells of extinct birds, including iconic giants such as the moa and elephant bird.

The achievement marks a major step towards drafting the genome of birds wiped out by human greed.

But the scientists are warning this does not mean an extinct species should, or even can, be resurrected in the style of Jurassic Park.

The team, led by Michael Bunce of Murdoch University in Perth, Western Australia, says the scientists isolated DNA from desiccated inner membranes in fossil eggshells, found in 13 locations in Australia, Madagascar and New Zealand.

Ancient genetic material was coaxed from the eggshell of the moa (Dinornis), a flightless cousin to the ostrich that reached up to four metres in height and was hunted to extinction by New Zealand's Maori by the late 18th century.

DNA was also extracted from the elephant bird (Aepyornis), like the moa and ostrich a species of ratite, said the study, published in the Proceedings of the Royal Society B.

Growing up to three metres high, the bird was wiped out by European colonisation of Madagascar by 1700.

Other successes were reported using eggshells from an Australian owl and a New Zealand duck of unknown date.

The oldest egg sample was from an emu (Dromaius novaehollandiae), some 19,000 years old.

It was procured by reducing the shell to powder, extracting the DNA with lab chemicals and then amplifying it using polymerase chain reaction, or PCR - a standard tool used by forensic scientists, for instance, in getting the famous "genetic fingerprint".

Bunce said the team extracted in each case only a tiny amount of DNA - just 250 base pairs, the "rungs" on the ladder-like genetic code, and this is less than a fraction of one per cent of the bird's genome.

"The point was proof of principle, to show that it can be done," he said in a phone interview with AFP.

"We didn't go out to get very long pieces of DNA. That's obviously the next step."

Bunce said the exploit would give palaeontologists a new window into creatures of the past.

Until now, DNA has been teased from bones - for instance, providing most of the genome of the Neanderthal, our enigmatic extinct cousins - and also from preserved hair.

Eggshells, overlooked as potential source material, turn out to be astonishingly robust at protecting DNA.

They are a tough biomineral matrix of calcium carbonate and are less prone than bones to infiltration by bacteria, which contaminates samples.

And they are also plentiful.

Archaeological sites have yielded harvests of eggs plundered from nests by early humans, eager to keep a store of protein at hand.

It could be possible to go back further than fossilised eggs that are older than 19,000 years, if the shells come from a permanently cold environment, the scientists hope.

But the team were unable to get DNA from far older samples in Australia, estimated at 50,000 years old, from an extinct megafaunal bird called Genyornis.

And eggs that are completely "mineralised" - in other words, like rock - over hundreds of thousands of years are not a likely option.

Bunce said resurrecting the moa, the elephant bird or the dodo, which was hunted to oblivion for food and hat feathers, remained "in the realm of science fiction".

"It's completely hypothetical, and frankly not a debate I really want to have...

"We can reassemble the genome to get an idea of what an extinct species looked like."